Search results for "Sample handling"
showing 5 items of 5 documents
Environment and excavation: Pre-lab impacts on ancient DNA analyses
2008
Ancient DNA (aDNA) analyses enjoy an increasing role in palaeontological, archaeological and archaeozoological research. The limiting factor for aDNA studies is the degree of DNA preservation. Our study on 291 prehistoric cattle remains from Europe, the Near East and North Africa revealed that DNA preservation is mainly influenced by geographic and climatic conditions. Especially in hot climates, the preservation of sample material is generally low. We observed that these specimens are prone to further degradation and contamination during and after excavation. We give a description of the main caveats and a short guideline for adequate sample handling in order to facilitate the cooperation …
Functional C1-inhibitor diagnostics in hereditary angioedema: Assay evaluation and recommendations
2008
Hereditary angioedema (HAE) is an autosomal dominant disease characterized by recurrent episodes of potentially life-threatening angioedema. The most widespread underlying genetic deficiency is a heterozygous deficiency of the serine protease inhibitor Cl esterase inhibitor (C1-Inh). In addition to low C4 levels, the most important laboratory parameter for correct diagnosis of HAE or angioedema due to acquired C1-Inh deficiency is reduced C1-Inh function (fC1-Inh). No direct recommendations about the assays for fC1-Inh or sample handling conditions are available, although this would prove especially useful when a laboratory first starts to offer assays on fC1-Inh for HAE diagnosis. In the p…
Identification of major histocompatibility complex class II-associated peptides derived from freshly prepared rat Langerhans cells using MALDI-PSD an…
2000
The isolation and identification is described of MHC class II-bound peptides derived from Langerhans cells. A combination of preparative micro-HPLC, MALDI-MS, Edman degradation was used for determining the amino acid sequence of MHC-associated peptides. Sample handling was crucial because fractions containing trace amounts of material require immediate storage at −80 °C to prevent peptide losses.
A Microassay for Measuring Glycogen in 96-Well-Cultured Cells
1996
Abstract This study describes a rapid, sensitive, and automated spectrophotometric enzymatic microassay that measures the intracellular glycogen of primary cultured hepatocytes and other cultured cells in 96-well plates and can be adapted for other samples that are transferred to these plates. The procedure involves in situ disruption of cells, followed by hydrolysis of glycogen into glucosyl units by fungal glucoamylase (exo-1,4-α- D -glucosidase, EC 3.2.1.3), and glucose determination with the glucose oxidase colorimetric method. The color intensity can be measured in conventional ELISA readers, and the data can be fed to an on-line computer for rapid processing. The advantages of this me…
Analysis of primary aliphatic short-chain monoamines by LC in water samples
2003
Abstract Several derivatization procedures with o-phthaldialdehyde-N-acetylcysteine (OPA-NAC) were compared for a rapid analysis of primary aliphatic short-chain monoamines in water samples by HPLC using a LiChorospher analytical separation column (100RP18 125 mm ×4 mm i.d., 5 μm). Both the solution and the solid-support assisted off-line derivatization on C18 SPE cartridges were inadequate options because of beginning degradation processes of the instable isoindol derivatives during their transfer to the analytical column. This problem was precluded with the on-column or solid-support assisted on-line derivatization. In the last mentioned procedure, the derivatization took place in a Hype…